November 26, 2015

Homework Help: Spinach

Posted by Sheryl on Monday, October 23, 2006 at 5:45pm.

I did a column chromatography on spinach and then a TLC. On the TLC I got five visible bands of colors. On the extracted yellow substance I got one yellow band (carotene) and on the green I also got only one green band. I thought there would be more bands on the green but maybe the chlorophyll was all that was in that band. Where did the other bands go?

Does it sound like I got reasonable results? Since there was no spinach at the stores, we used some that was growing wild under my deck and it was very dry.

Thanks from Sheryl

It isn't clear from your description what was extracted.

Guess sometimes I could look in the book rather than spend an hour on the internet. We were extracting carotene and chlorophyll a and b. Looks like I did my experiment okay. There seems to be a visible as well as a not very visible band in the green extract. Sorry to bother.


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