Sometimes, certain enzymes are associated with a particular organelle of the cell. Many times scientists have had to use ______ centrifugation to separate different constituents of the cell. Following this procedure, you aliquot the fractions into separate test tubes, each correlating to a particular layer (or fraction) of your centrifugation end product. One way to help isolate an enzyme from among all the fractions collected is to assay for enzyme _. This will help delineate which fraction the enzyme is in, and even some of its properties.

Question

Sometimes, certain enzymes are associated with a particular organelle of the cell. Many times scientists have had to use ______ centrifugation to separate different constituents of the cell. Following this procedure, you aliquot the fractions into separate test tubes, each correlating to a particular layer (or fraction) of your centrifugation end product. One way to help isolate an enzyme from among all the fractions collected is to assay for enzyme _. This will help delineate which fraction the enzyme is in, and even some of its properties.

a. differential, activity
b. micro, presence
c. micor, products
d. swing-bucket, activity

I think the answer is "A" but I don't really have any solid reason for it.

Blank 1:

http://en.wikipedia.org/wiki/Differential_centrifugation

Blank 2:
http://en.wikipedia.org/wiki/Enzyme_activity

Answer 1

Blank 1: The technique described here involves separating different constituents of the cell using centrifugation. To determine which option fits in Blank 1, we can look at the given Wikipedia link for "Differential centrifugation." The link explains that differential centrifugation is a method used to separate different cellular components based on their size and density. This technique involves multiple rounds of centrifugation at increasing speeds, resulting in the separation of organelles and other cellular components into different fractions. Therefore, the correct answer for Blank 1 is "a. differential."

Blank 2: To determine which option fits in Blank 2, we can look at the given Wikipedia link for "Enzyme activity." The link explains that enzyme activity refers to the ability of an enzyme to catalyze a specific reaction. To isolate an enzyme from among all the fractions collected, one can assay for enzyme activity. By measuring the enzyme's activity, it is possible to determine in which fraction the enzyme is present and even gain some insight into its properties. Therefore, the correct answer for Blank 2 is "b. activity."

Based on the explanations provided above, the correct answer for this question is "a. differential, activity."